Title: : Effect of Salvia hispanica L (chia) seed on hemostasis and thrombosis in an experimental model of Metabolic Syndrome

Abstract

Introduction: The metabolic syndrome is a clinical entity represented by the co-occurence of abdominal fat, dyslipidemia, hypertension, insulin resistance, glucose intolerance, low-grade chronic inflammation state, endothelial dysfunction, prothrombotic state, among others. A procoagulant and hypofibrinolytic state has been identified; mainly associated of the inflammatory state and dyslipidemia. Salvia hispanica L. (chia) seed is one of the richest botanical sources of α-linolenic acid (ALA, 18:3 n-3) and it has generated considerable research interest in recent years and its effects on these disorders are very little known. Objective: To study the effect of the chronic administration of a sucrose rich diet (SRD) on hemostasis and thrombosis in normal rats. To evaluate the effects of Salvia hispanica L. seed as dietary manipulation. Methods: Male Wistar rats were fed a SRD for 3 months. Half of the animals continued with the SRD until month 6, the other half was fed a SRD in which the fat source, corn oil, was replaced by chia seed from month 3 to 6 (SRD+chia). Another group consumed a reference diet all the time. We analyzed: Hemogram: red blood cells, white blood cells, hemoglobin concentration, hematocrit and platelets were determined in hematological counter (SYSMEX KX-21). Clotting time (CT), Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen and lipids plasma levels were assessed by commercial kits. Blood pressure was measured using CODA TM Monitor of tail-cuff non-invasive system. Results: The study showed that chia seed administered to SRD-fed rats: a- increase platelet levels (P<0.05), and the CT, PT, APTT, TT, which are significantly lower in SRD-fed animals, b- reduce plasma fibrinogen levels (P<0.05), reaching reference values. This was accompanied by a normalization of dyslipidemia and blood pressure. Conclusion: Our findings suggest that chia seed has beneficial effects on the hypercoagulability and hypofibrinolysis present in SRD-fed animals.

Biography

María Eugenia Oliva. PhD in Biological Sciences. Researcher of National Council for Scientific and Technical Research (CONICET) and Associate Professor of Biological Chemistry cathedra. Faculty of Biochemistry and Biological Sciences, National University of Litoral. Santa Fe, Argentine.